Histology

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Before you begin this section in lab, please read the page on the Use of the Microscope. This will allow you to get the best view of the specimens and help us take care of our investment.

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Tissue Preparation

In order to prepare tissue for viewing under the light microscope, the following steps are usually taken:

· A small tissue sample is taken and "fixed" by infiltrating it with a preservative. This fluid is usually water-based.

· The fixed material is gradually passed stepwise through a series of solutions, each with increasing concentrations of alcohol.

· The material, impregnated with alcohol, is gradually passed through a series of solutions with increasing amounts of organic solvent.

· Paraffin is substituted for the organic solvent in a stepwise manner.

· Paraffin blocks are solidified, each about the size of a mini ice cube.

· Serial sections of the tissue in paraffin are made using a microtome.

· Strips of serial sections are placed on a slide and heated slightly to help them adhere.

· Slides with specimens are placed in stains, selected to show prominent characteristics of the sample.

· Mounting medium is place on the slide and a coverslip is applied.

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Worksheet for Histology
Tissue or Organ	Slide	Text Pg.	Function or Definition
Epithelium	none	none	D:
Simple Epithelium	none	none	D:
Squamous	-	-	F:
Cuboidal	-	-	F:
Columnar	-	-	F:
Stratified Epithelium	none	none	D:
Squamous	-	-	F;
Transitional Epithelium	-	-	F:
Pseudostratified Epithelium	-	-	F:
Connective Tissue	none	none	D:
Areolar	-	-	F:
Adipose	-	-	F:
Compact Bone	-	-	F:
Hyaline Cartilage	-	-	F:
Elastic Cartilage	-	-	F:
Dense Regular	-	-	F:
Dense Irregular	-	-	F:
Blood	-	-	D:
Erythrocyte	-	-	F:
Neutrophil	-	-	F:
Monocyte	-	-	F:
Lymphocyte	-	-	F:
Muscle Tissue	none	none	F:
Skeletal Muscle	-	-	F:
Cardiac Muscle	-	-	F:
Smooth Muscle	-	-	F:
Nervous Tissue	-	-	F:
Neurons in Oxtail Slide	-	-	F:
Other Slides	none	none	-
Esophagus	-	-	F:
Kidney	-	-	F:
Large Intestine	-	-	F:
Lung	-	-	F:
Liver	-	-	F:
Salivary Gland	-	-	F:
Stomach	-	-	F:
Small Intestine	-	-	F:
Pancreas	-	-	F:
Ovary	-	-	F;
Testes	-	-	F:
Tongue			F;
Artery and Vein	-	-	F:
Scalp (skin)	-	-	D:
Sebaceous Gland	-	-	F
Sweat Gland	-	-	F